Western Blotting for the Non-Expert

1. Western blotting: How it began
2.  Basics of Western Blotting

 

Basic Calculations in Western Blotting

Choosing The Right Antibody For Western Blotting

 

1. Sample Preparation

 

1. Blocking membranes at places that do not have transferred proteins

 

1. A Basic Method for Western Blotting

 

1. Trouble Shooting Western Blotting

 

1. Using Biotin to demonstrate western and dot blotting in University Practical Classes-

 

A Guide To Instructors

 

1. Nitrocellulose Membrane Art

 

1. The Use Of A Harmless Invisible Ink To Send Secret Messages On Nitrocellulose Membranes And As A Substitute For The Harmful Radioactive Ink Used As A Marker In ECL Membrane Assays

 

1. Fingerprinting On Nitrocellulose And Polyvinylidene Difluoride Membrane

 

13.  13. Spectrometric Determination Of Protein For Electrophoresis

 

14.  14. Concentrating Proteins For Western Blotting

 

15.  15. Dissolving Proteins Using Lysis Buffer

1. Blotting And Detecting Glycosaminoglycans

 

1. Passive Blotting From Tissue Slices

 

1. Localizing Proteins by Tissue Printing

 

1. Ultrasound Blotting

 

Obtaining Several Blots From A Single SDS-PAGE Gel By Passive Transfer

 

1. Diffusion Blotting From SDS-PAGE Gels Supported By Solid Plastic

 

1. Dot-Immunobinding

 

1. Simultaneous Immunoblotting Analysis With Activity Gel Electrophoresis

 

1. Protein Blotting By Centrifugation

 

1. Blotting And Immunostaining Of Multiple Antigenic Peptides

 

1. High Molecular Weight Protein Blotting Using Agarose Gel Electrophoresis

 

1. Cetyltrimethylammonium bromide PAGE And Eastern Blotting

 

Purification Of Autoantibodies Bound To An Autoantigen Immobilized On A Membrane Strip

 

1. Miniaturized Blotting

 

1. Affinity Immunoblotting

 

Transferring Coomassie Blue Stained Proteins from Polyacrylamide Gels To Transparencies

 

1. Protein Transfer From Immobilized pH Gradient Gels

 

1. SDS PAGE To Immunoblotting In Sixty Minutes

 

Western Blot Analysis Of Protein-DNA Complexes Formed DuringGel Shift Experiments

 

1. Electrophoretic Transfer Of High And Low Molecular Weight Proteins To Membranes Using Heat

 

1. Grid-immunoblotting

 

1. Far-western Blotting

 

1. Native Electrophoresis And Western Blot Analysis

 

1.  Efficient Electroblotting Of Low Molecular Weight Protein After Staining With Coomassie

 

Avoiding Non-specific Binding Of Secondary Antibodies In Immunoblotting By Double-blotting

 

1. A Brief Introduction To Other Protein Blotting Methods

 

Quantitative Computerized Western Blotting In Detail

 

    43. Common Problems In Electrophoresis